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Käte kõhklus diabeediraviga


Isotope Effects: Experimental • An isotope effect is measured to determine if the bond at which the isotopic substitution has been made changes in some manner during the rate-limiting step. • The isotope effect is expressed as a ratio of rate constants: the rate constant for the reaction with the natural abundance isotope over the rate constant for the reaction with the altered isotope.



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Kinetic isotope effect measurement at natural abundance is a simple general method for measuring kinetic isotope effects (KIE) for chemical reactions performed with materials of natural abundance. This technique for measuring KIEs overcomes many limitations of previous KIE measurement methods.
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In fact, binding and subsequent release of a ligand might be the sole function of the macromolecule (example myoglobin binding oxygen). Binding is the first step necessary for a biological response (with the exception of visual transduction in which photo-induced isomerization of rhodopsin initiates the response).
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